Our Systems
SKI LODGE
The DNA sequence (i.e., gene cDNA) can be introduced as a single copy, regulated by five tissue-specific promoters.
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We generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the C. elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by five different tissue-specific promoters.
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SKI PLACE
Any gene (with regulatory sequences) can be cloned into the pSKI plasmid by traditional cloning or Gibson Assembly® and then inserted as a single copy into any chromosome.
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We generated a single-copy knock-in system: one plasmid to target all chromosomes in C. elegans (SKI PLACE). This system uses the pSKI plasmid (with 47 restriction sites), which facilitates the insertion of a gene of interest at specific harbor loci on each chromosome. The pSKI plasmid contains two synthetic 900-bp homology arms that recombine with the SKI PLACE cassettes. This system also uses a single crRNA guide.
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